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. 2016 Dec 3;8(3):5057–5068. doi: 10.18632/oncotarget.13789

Figure 6. MiR-433-3p increases chemosensitivity of glioma cells to temozolomide (TMZ) by targeting CREB.

Figure 6

A. The cells transfected with scramble or miR-433-3p mimics were treated with different concentrations of TMZ solution, and cell survival was assayed by MTT 48 h later. *P < 0.05 versus Scramble. B. The cell proliferation in the presence of TMZ (100 μM) was tested by MTT every 24 h in both U251 and U87 cells overexpressing scramble and miR-433-3p. *P < 0.05 versus Scramble. C. U251 and U87 cells were co-transfected with miR-433-3p mimics and pCMV6/CREB or their control oligomers and vectors in the presence of TMZ (100 μM). Then flow cytometry analysis was performed to detect cell apoptosis rates in every group. *P < 0.05 versus TMZ + Scramble + pCMV6. #P < 0.05 versus TMZ + miR-433-3p + pCMV6. D. Growth curve of U87 xenograft tumors and mice treated with miR-433-3p and TMZ at the end of the study. *P < 0.05 versus Scramble+TMZ. A–C. experiments were repeated three times. All data are shown as mean ± SD.