Figure 7. CUG2 activates Akt and MAPKs, which are dependent on TGF-β signaling, except p38 MAPK.

A. Activation of Akt, ERK, JNK and p38 MAPK in A549-CUG2 and BEAS-CUG2 cells was compared with those in their control cells by immunoblotting using their corresponding phospho-specific antibodies. B. A549-CUG2 and BEAS-CUG2 cells were treated with EW-7197 at the dose (1.25 μM) for 24 h. Activation of Akt, ERK, JNK and p38 MAPK was examined by immunoblotting.