Figure 5. Effects of mTOR and PI3K inhibitors on constitutive release of angiogenic mediators by stromal cells.

The effects of mTOR (rapamycin, temsirolimus) and PI3K inhibition (GDC-0941, 3-MA) on in vitro constitutive mediator release was investigated for 10 different stromal cell populations. Cell supernatants were harvested from stromal cell cultures before confluence was reached. Levels of each mediator were determined using ELISA, and relative values (levels in drug-treated cultures divided by levels in corresponding control cultures) were log(2) converted. Squares are omitted where no detectable levels of mediators were measured in control/treated cultures. The various stromal cell populations examined are indicated in the right column.