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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Leuk Res. 2017 Jan 12;55:23–32. doi: 10.1016/j.leukres.2017.01.007

Figure 4. BTdCPU induces phosphorylation of eIF2α and promotes cell death in MM cells.

Figure 4

(A) Levels of the heme-regulated inhibitor kinase (HRI) relative to Tubulin were assessed by Western blotting of lysates of Dex-sensitive (MM1.S) and Dex-resistant (MM1.R, RPMI 8266, U266) MM cells. (B) MM1.S or MM1.R cells were treated with 10 μM BTdCPU for 0, 4 or 8 hours and immunoblotted for expression of phospho-eIF2α, total eIF2α, CHOP and Tubulin. (C) MM1.S or MM1.R cells were treated with 10 μM BTdCPU for 0 or 4 hours. Expression levels of total CHOP mRNA were quantitated relative to GAPDH. (D) Dex sensitive and resistant cell lines were treated with 0 to 20 μM BTdCPU for 48 hours. Cell viability was measured using an MTS assay and relative survival levels are graphed. (E,F) MM1.S or MM1.R cells were co-cultured with bone marrow stroma (E) or HUVEC (F) with or without 10 μM BTdCPU for 24 hours and apoptosis was measured with AnnexinV/PI. **p <0.01.