Figure 2.

The effect of AA on redox homeostasis in prostate cancer and normal cells. A, Prostate cancer and normal epithelial cells were treated with AA and then incubated with Amplex Red. The extracellular H₂O₂ concentration was calculated by a standard curve and normalized to the cell number. B, Concentrations of mitochondrial superoxide in PC3 and PZ cells were measured by MitoSox Red after treatment with AA and IR as indicated. Cellular fluorescence intensity was detected by fluorescence microscopy and a fluorescence microplate reader. Mitochondria were visualized by staining with Mitotracker Green. Microplate readings were normalized by protein levels. n.s, no significance detected. All the analyses were performed using one-way ANOVA with post-hoc Tukey HSD test.