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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Cancer Res. 2017 Jan 20;77(6):1345–1356. doi: 10.1158/0008-5472.CAN-16-0785

Figure 6.

Figure 6

Modulation of SIRT3 due to AA-mediated RelB regulation. A, After AA treatment, the expression of SIRT1, SIRT3, RelB, and MnSOD in PC3 and PZ cells was measured by western blots. B, Protein levels of SIRT1 and SIRT3 after manipulation of RelB expression in LNCaP and PC3 cells were determined by western blots. C, After AA treatment, mRNA levels of SIRT3 in PC3 cells and PZ cells were determined by RT-PCR. D, Protein–DNA complexes were extracted from the AA-treated or untreated PC3 and PZ cells and then immunoprecipitated using a RelB antibody. The SIRT3 promoter fragment containing a NF-κB element was amplified by q-PCR with specific primers, which were normalized by their input controls containing relevant unprecipitated chromatin. E, After AA and radiation treatment, the expression of RelB-SIRT3 in PC3 and PZ cells was measured by western blots. One-way ANOVA with post-hoc Tukey HSD test was performed for comparison of multiple groups in cells.