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. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Cancer Res. 2017 Jan 23;77(6):1383–1394. doi: 10.1158/0008-5472.CAN-16-2012

Figure 3.

Figure 3

FFA promotes TGF-β-induced invasion and migration by activating ERK and USP9x. A, In vitro invasion assay performed on MCF-7 cells that were treated with BSA or PA in the presence or absence of AZD6244 or WP1130 with or without TGF-β1 for 20 hours. Each column (right panel) represents the mean (± SD) results of two independent experiments. *p<0.05 versus BSA; #p<0.05 versus PA. (B) MCF-7 and (C) MDA-MB-231 cells were scratched with a 20-µl pipette tip and then incubated with BSA, PA, PA plus AZD6244 or WP1130 in the absence or presence of TGF-β1. Migrating cells were photographed under a phase contrast microscope. The percentage of the wound closed was quantified from three independent replicates and is expressed as mean ± SD. *p<0.05 vs BSA/TGF-β; #p<0.05 vs PA/TGF-β.