Figure 2. Bone pain and SN activation in JJN3-injected mice.
A. Mechanical allodynia. * p<0.05, ** p<0.01 vs sham mice (mean ± SE, n=16). Arrow indicates a single intraperitoneal injection of Baf A1 (25µg/kg) or vehicle (0.1% DMSO in PBS).
B. Thermal hyperalgesia. * p<0.05 vs sham mice (mean ± SE, n=16). Arrow indicates a single intraperitoneal injection of Baf A1 (25µg/kg) or vehicle (0.1% DMSO in PBS).
C. Sprouting of CGRP+ SNs in tibiae.
D. Quantitative data of C. * p<0.05 vs sham mice (mean ± SE, n=16).
E. Acidification of bone marrow shown by acridine orange accumulation.
F. Time-course of inhibition of mechanical allodynia, following a single intraperitoneal injection of Baf A1 (25µg/kg) or vehicle (0.1% DMSO in PBS) (arrow in A and B). * p<0.05 vs vehicle-treated JJN3-injected mice (mean ± SE, n=8).
G. Thermal hyperalgesia, following a single intraperitoneal injection of Baf A1 (25µg/kg) or vehicle (0.1% DMSO in PBS) (arrow in A and B). * p<0.05 vs vehicle-treated JJN3-injected mice (mean ± SE, n=8).
H. Excitation of sensory nerves determined by pERK1/2 and pCREB expression in DRG in sham and JJN3-injected mice.