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. 2017 Jan 29;8(8):12620–12636. doi: 10.18632/oncotarget.14896

Figure 1. An established protocol for short RNA-oligoprobe labeling in FFPE tissue sections.

Figure 1

A. GAPDH oligonucleotides were tested in the cell line HEK 293. Single fluorescent spots, each representing one single RNA molecule, are clearly visible. B. Normal deparaffinization procedure shows high background noise in FFPE islet sections even without probes (No Probes) and does not allow clear distinction of probe signal and background, when GAPDH oligonucleotides were added C.; Standard Protocol. Modification of the deparaffinization and post-hybridisation protocol leads to background reduction and increased signal intensity of GAPDH oligonucleotides D.; Modified Protocol. RNA Probes are labeled with Quasar 570 (red) and nuclei were stained with DAPI (blue), scale bar depicts 10µm.