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. 2017 Jan 29;8(8):12620–12636. doi: 10.18632/oncotarget.14896

Figure 5. Viral RNA within an islet of a T1D patient.

Figure 5

A. The original probe set CVB_1 was complemented with two additional sets (CVB_2 and CVB_3). Scheme of custom-designed probe sets annealing throughout the viral genome. Arrows show localization of CVB_1, lines show the positions of CVB_2 and CVB_3 probe sets. B., C. Representative images of donors E375 and E514 form the UK cohort and of donor 3626 from the nPOD cohort. Virus RNA was found within the endocrine area (B) and outside the islets (C) shown by the co-staining of viral RNA probes (combination of CVB_1, CVB_2 and CVB3) (red) and insulin (Green). Tissues were first probed for viral RNA, analyzed and then stained for insulin. Nuclei were visualized by DAPI staining (blue); scale bar depicts 10µm.