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. 2017 Jan 3;8(8):12764–12774. doi: 10.18632/oncotarget.14463

Figure 9. Ibrutinib's synergy with HHT is dependent of FLT3.

Figure 9

MV4-11 cells were transduced with 3 different SiRNAs targeting FLT3, STAT5 or a non-targeting siRNA control. The reduction of FLT3 or STAT5 expression was confirmed by qPCR and western blotting. (A) On day 3 to 5 post-transduction, cells were treated with HHT at concentrations previously shown to synergize with ibrutinib for 24 h. The CIs at the ED50, ED75 and ED90 were presented. (B) Cells were treated with 0.625 μM ibrutinib and 2 nM HHT for 48 h. Cell growth and viability was determined by a MTT assay. (C) The data are presented as mean inhibition rates ± SD from at least three independent experiments.