Figure 2. SKLB-178 induces G0/G1 phase arrest and apoptosis in NSCLC cells.
(A) Influence of SKLB-178 and Gefitinib on cell cycle progression in NSCLC cells. Representative images for three NSCLC cell lines treated with indicated concentrations of SKLB-178 or Gefitinib are shown on the left, and the percentages of the cell cycle are also presented on the right. Data shown in the histogram are means ± SD from three independent experiments. (B) PC-9 and H1975 were cultured in the presence of SKLB-178, Gefitinib or vehicle, followed by apoptosis detection using Annexin V/PI co-staining. The assays were performed in triplicate, and the representative images are shown in the graph (left). The percentage of Annexin V-positive cells is quantified for apoptotic rate statistics. ***P < 0.001 vs vehicle; ns, no significant difference.