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. 2017 Jan 11;8(8):12843–12854. doi: 10.18632/oncotarget.14597

Figure 4. SKLB-178 inhibits angiogenesis through blockade of VEGFR signaling pathway.

Figure 4

(A) SKLB-178 inhibited migration, invasion, and tube formation of endothelial cells. The concentration of SKLB-178 used in the HUVECs assay were all 1 μM. The index lines in the migration assay indicate the original scratch position. Scale bars represent 100 μm. (B) SKLB-178 inhibited the phosphorylation of VEGFR and its downstream signaling ERK in VEGF-stimulated HUVECs. HUVECs were serum starved overnight, and then incubated with vehicle or serial dilutions of SKLB-178 for 2 h, followed by treatment with 50 ng/mL VEGF for 15 min. Cells were then lysed for western blot assay. (C) SKLB-178 inhibited zebrafish embryonic angiogenesis. Fluorescent images were taken after treatment with indicated agents. Scale bar represents 100 μm.