Figure 3. The 3′RR partially rescues CSR in trans on a 3′RR-cis-deficient IgH allele.

A. In vivo IgH^a allele CSR in Δ3′RR^a/wt^b mice: IgG2a (top) and IgA^a (bottom) serum levels from homozygous Δ3′RR, Δ3′RR^a/wt^b, wt^a/b and wt^b/b mice. Each point represents one mouse, mean is shown (≥ 3 independent experiments). Mann-Whitney test for significance. B. In vitro IgH^a allele CSR in Δ3′RR^a/wt^b cells: IgG2a (top) and IgE^a (bottom) levels in supernatant from Δ3′RR, Δ3′RR^a/wt^b, wt^a/b and wt^b/b stimulated B-cells. Each point represents one stimulation from one mouse, mean is shown (≥ 3 independent experiments). Mann-Whitney test for significance. C. ELISpot specific for IgG2a (top) and IgE^a (bottom) were performed on in vitro stimulated B-cells from Δ3′RR, Δ3′RR^a/wt^b, wt^a/b and wt^b/b. Data shown are from 3 independent experiments with at least 2 mice. Mean +/- s.d. is shown. Mann-Whitney test for significance.