Figure 4. The 3′RR partially rescues SHM in trans on a 3′RR-cis-deficient IgH allele.

A. Mutation frequency in 3′J_H4 intron from Peyer's patches (B220⁺/GL7⁺) in Δ3′RR/Δ3′RR mice and in Δ3′RR^a and wt^b alleles from Δ3′RR^a/wt^b mice. Mean +/- s.d. is shown. Mann-Whitney test for significance. B. Mutation frequency as in (A), but with each point representing an individual mouse in order to evaluate the dispersion of the results when SHM is measured in groups of mice with homogeneous genotype. C. Graph of mutation frequencies along the sequenced J_H4 flanking region in homozygous Δ3′RR mice (top) and in Δ3′RR^a (middle) and wt^b (bottom) alleles from Δ3′RR^a/wt^b mice. D. Mutation frequencies in 3′J_H4 intron in Peyer's patches B220⁺/GL7⁺/IgM⁺ cells from homozygous Δ3′RR and Δ3′RR^a/wt^b heterozygous mice. Data were obtained from at least 3 mice per group and clonally related sequences were counted only once. Khi2 test for significance.