Figure 2. Cerdulatinib induces apoptosis in CLL in association with MCL-1 down-regulation and PARP cleavage.

A. Cerdulatinib induces apoptosis. Apoptosis was assessed by annexin V/7-AAD staining following cerdulatinib treatment for 48 hrs. Three representative cases are shown. The percentage of early apoptotic annexin-Vhi/7AAD low population in the bottom right quadrant is indicated. B. Dose response of 8 CLL samples at indicated concentrations of cerdulatinib post 48 hr of treatment. Data presented represent mean ± SE of apoptosis. ***, P < 0.001. C. Immunoblots of MCL-1 and PARP. Following cerdulatinib treatment for 48 hrs at indicated concentrations, MCL-1 and PARP1 cleavage were measured by Western blot in whole cell lysates. GAPDH was included as the loading control.