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. 2017 Jan 16;8(8):12983–13002. doi: 10.18632/oncotarget.14677

Figure 1. Expression of PHD3 in HCC patients.

Figure 1

(A) IHC analysis of PHD3 expression in 81 HCC tissues and paired ANLTs. Representative images were taken. Scale bar, 500 μm (upper) or 100 μm (lower). (B) IHC scoring was displayed by a scatter plot, with the median indicated. (C) Western blotting of tumor tissue and paired ANLTs in 24 randomly selected HCC cases with β actin as the loading control. (D) The relative densities of PHD3 protein in 24 cases were calculated and shown by a scatter plot, with the mean and SEM indicated. (E) the mRNA level of PHD3 was analysed by Real-time PCR in 40 randomly selected HCC tissues and paired ANLTs. The vertical axis means the logarithm base 2 of the relative expression of PHD3 in tumor tissue compared with ANLTs. Bar value ≤ –1 and ≥ 1 indicate that the expression of PHD3 is decreased and increased in tumors, respectively. (F) The ΔCt value was presented with a box plot reporting the median values and the interquartile range.