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. 2016 Dec 31;8(8):13085–13098. doi: 10.18632/oncotarget.14393

Figure 1. Establishment of ex vivo culture of primary lymphoma cells using CAF.

Figure 1

A. Pathological specimens of lymph node samples of intractable DLBCL patients (#1 and #2). HE staining (a), L26 immunostaining (b), split FISH assays for BCL2 probes (c) and for MYC probes (d) are shown. B. Viability of lymphoma cells at 48 h after initiation of co-culture with or without CAF. A bar graph of relative cell viability under each culture condition is shown. Each point represents the mean value taken from three representative independent experiments. Error bars indicate SEM. Asterisks indicate the P value as follows; * P < 0.05; ** P < 0.01; **** P < 0.0001. C. Long-term ex vivo culture of lymphoma cells. Lymphoma cells of patients #1 and #2 were cultured with or without CAF. Each point represents the mean value taken from three representative independent experiments. Error bars indicate SEM. D. ATP levels were measured in lysates from lymphoma cells (#2) cultured with or without CAF. Each point represents the mean value taken from three representative independent experiments. Error bars indicate SEM. Asterisks indicate the P value as follows; ** P < 0.01 E. Whole cell lysates of tumor cells (#2) were obtained at 48 h after initiation of the co-culture with or without CAF. Immunoblotting was performed for HK2, PDK1 and LDHA. Tubulin was blotted as a loading control.