Figure 6. Nrf2 knockdown abolishes miR-141-meidated RPE cytoprotection against UV.

miRNA-141-expressing RPEs (“L1/2”) were infected with lentiviral Nrf2 shRNA (“shNrf2-A/-B”, with non-overlapping sequence) or scramble control shRNA (“shSCR”), expression of Nrf2 mRNA (A) and (B) and listed proteins (C) and (D) were shown. Above cells were also irradiated with UV (30 mJ/cm²) and cultured 24 hours, and cell viability was tested by MTT assay (E) and (F). Nrf2 expression (vs. loading Erk1/2) was quantified (C and D). For each assay, n=5. Experiments in this figure were repeated three times to insure consistency of results. *p<0.05 vs. “shSCR” cells.