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. 2017 Jan 18;8(8):13253–13263. doi: 10.18632/oncotarget.14710

Figure 7. SOX2 can rescue effect of miR-625 in malignant melanoma.

Figure 7

A. Cell proliferation was measured by the WST assay. A375 cells were transfected with miR-625 mimics, NC mimics, SOX2, or Vector control, at 24 hours after transfection. The WST assay was performed every 24 hours for 4 days. Results are means of three independent experiments ± S.D. B. M14 cells were transfected with miR-625 mimics, NC mimics, anti-625, or anti-NC at a final concentration of 100 nM and, at 24 hours after transfection. The WST assay was performed every 24 hours for 4 days. Results are means of three independent experiments ± S.D. C. Representative photographs of migratory A375NC mimics+Vector, A375miR-625+Vector, and A375miR-625+SOX2 cells on the membrane (magnification, 100×). D. Average number of migratory A375 cells. E. Representative photographs of migratory M14NC mimics+Vector, M14miR-625+Vector, and M14miR-625+SOX2 cells on the membrane (magnification, 100×). F. Average number of migratory M14 cells. The data represent the mean ± S.D. of three independent experiments. G. Representative images of spheres as indicated. Scale bar = 50 μm. H. Average number of spheres. I. Measurement of the size of melanoma spheres. (*P < 0.05, **P < 0.01).