Skip to main content
. 2017 Jan 5;8(8):13344–13356. doi: 10.18632/oncotarget.14527

Figure 2. PDHA1 gene mutation identification.

Figure 2

(A) The targeting area of PDHA1 extron1 was amplified from genomic DNA and the PCR fragments were sequenced to identify mutation. (a) Sequence chromatograms for the mutant type. (b) The deleted mutant bases are shown in green shade including 45 bases in the exon (with underline) and 27 bases in intron. (B) cDNA sequence from reverse transcriptase-polymerase chain reaction (RT-PCR). (a) Sequence chromatograms (b) Wild and mutant cDNA sequence comparison. (c) cDNA bases and the coding sequence (with underline) are shown. (C) The expressions of PDHA1 protein were examined by western blotting.