Figure 5. HnRNP-L overexpression enhances migration in UM-UC-3 and EJ cells.

(A) Migration of cells was assessed by the wound closure assay. After 48 h, the wound was nearly closed by hnRNP-L transfected cells and was wider with sh-hnRNP-L transfected cells. Vertical bars indicated the mean distance of cell migration as compared with the negative control; (B) In the transwell assay, migration was quantified by cells migrating through the bottom chamber. The cell numbers were significantly increased in hnRNP-L transfected cells and were decreased in sh-hnRNP-L transfected cells; (C) Statistics analysis of the mean migration cell numbers as compared with the negative control. All experiments were performed three times independently, *p < 0.05; **p < 0.01; ***p < 0.001; (D) In both cell lines. EMT markers were modulated by hnRNP-L expression. Epithelial markers (E-cadherin and β-catenin) and the tight junction proteins (ZO-1 and caludin-1) were promoted when hnRNP-L knockdown, whereas mesenchymal markers (Vimentin) and the repressor of E-cadherin (snail, ZEB1) were down-regulated; (E) While opposite results showed in hnRNP-L overexpressed cells. GAPDH served as loading control.