Skip to main content
. 2017 Jan 13;8(8):13666–13677. doi: 10.18632/oncotarget.14623

Figure 4. MAP4K3 down-regulation requires the cooperation of miR-199a-5p and let-7c, and MAP4K3 promotes HCC cell migration and invasion.

Figure 4

(A, B) Western blot assays of endogenous MAP4K3 protein levels in HepG2 cells transfected with the miR-199a-5p, let-7c, miR-199a-5p and let-7c agomirs, negative control or miR-199a-5p and let-7c inhibitors. (C, D) Western blotting of MAP4K3 protein expression in HepG2 cells infected/transfected with lenti-MAP4K3, lenti-control, MAP4K3-siRNA or negative control-siRNA. (E, F) Infection/transfection of HepG2 cells with lenti-MAP4K3, lenti-control, MAP4K3-siRNA or negative control-siRNA was performed to investigate the effects of MAP4K3 on HCC cell migration and invasion. Representative images are presented.