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. 2017 Jan 13;8(8):13666–13677. doi: 10.18632/oncotarget.14623

Figure 5. Restoration of MAP4K3 promotes miR-199a-5p- and let-7c-mediated migration and invasion of HCC cells.

Figure 5

(A) Expression vectors were generated by cloning miR-199a-5p and let-7c into a lentiviral vector. (B) qRT-PCR assays of mature miR-199a-5p and let-7c expression in HepG2 cells infected with a lentiviral vector expressing miR-199a-5p and let-7c or negative control. (C) Western blot analysis of MAP4K3 expression in HepG2 cells or HepG2- miR-199a-5p-let-7c stable cells with or without MAP4K3 reintroduction. (D) Transwell migration assays of HepG2 cells or HepG2-miR-199a-5p-let-7c stable cells with or without MAP4K3 reintroduction. Representative images are shown. The values are the mean ± SEM; asterisks indicate significance. (E) Transwell invasion assays of HepG2 cells or HepG2-miR-199a-5p-let-7c stable cells with or without MAP4K3 reintroduction.