Figure 3. Effect of conditioned medium from MCF-7 cells on normal mammary fibroblasts spheroids in 3D collagen matrices.
(A) Representative images of fibroblast spheroids exposed to conditioned medium from MCF-7 cells transfected with an empty vector (WT) or cells overexpressing Fibulin-2 (Fib.2), ADAMTS-5 (ADAMTS-5) or both (Fib.2 + ADAMTS-5) at the indicated times. Medium without cultured cells was employed as control medium. (B) Measure of the branching (invasive area) by the fibroblasts spheroids as fold-changes increases at the indicated times.