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. 2017 Jan 13;8(8):13770–13781. doi: 10.18632/oncotarget.14634

Figure 4. Quiescent MCF7 cells are more sensitive to compound cytotoxicity than quiescent MCF10A cells.

Figure 4

(A) Quiescent states in cancer versus normal cells. Cancer cells featuring autonomous cell proliferation presumably have a shallower and unstable quiescent state compared to normal cells. (B, C) Compound cytotoxicity time course in quiescent MCF7 vs. MCF10A cells. Serum starvation-induced quiescent MCF7 and MCF10A cells were treated with ergosterol peroxide (B) or ganodermanondiol (C) at 10 and 20 μg/ml as indicated. The relative live cell count (y-axis) was determined as in Figure 1B, at the indicated day (x-axis) during the course of treatment. Error bar indicates the standard error of the median (of four replicates). (D) Induced apoptosis in quiescent MCF7 cells. Serum starvation-induced quiescent MCF7 cells were treated with ergosterol peroxide or ganodermanondiol at 20 μg/ml or DMSO vehicle control. Cells were assayed for mitochondrial membrane potential (TMRE) and externalization of inner membrane phospholipids (Annexin V) after 2 days of treatment.