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. 2017 Jan 13;8(8):13792–13804. doi: 10.18632/oncotarget.14637

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Copyright: © 2017 Jelinic et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) EMSY-overexpressed and/or PKA-depleted 293T cell lysates were immunoprecipitated with V5 antibody and blotted with PKA-phosphosubstrate (PKA-p-sub) antibody. The left side panels demonstrate EMSY overexpression and PKA knockdown by siRNA. The right panels show V5-immunoprecipitation (V5-IP) and detection of PKA-targeted EMSY phosphorylation using the PKA-p-sub antibody. Overexpressed EMSY is detected with the V5 antibody (V5^EMSY). (B) EMSY and PKA co-expressed 293T cell lysates were immunoprecipitated with V5 antibody and blotted with PKA-p-sub. The left panels show EMSY and FLAG-tagged PKA overexpression. The right panels show V5-immunoprecipitation (V5-IP) and detection of PKA-targeted EMSY phosphorylation using the PKA-p-sub antibody. Overexpressed PKA is detected with FLAG (FLAG^PKA) antibody. For (A) and (B) IgG heavy chain (V5^IgG) ponceau staining and anti-β-Actin served as loading controls.