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. 2017 Jan 13;8(8):13832–13845. doi: 10.18632/oncotarget.14640

Figure 4. CEBPD is involved in metformin-induced autophagy.

Figure 4

(A) Huh7 cells were transfected with pcDNA3/HA (Ctrl) or pcDNA3/HA-CEBPD (CD) expression vectors or (B) infected with lentiviruses encoding shLacZ (shC) or shCEBPD (shCD) and then treated with or without metformin (Met, 5 mM). The lysates and total RNA were harvested for Western blot analyses and RT-PCR, respectively. (C) GFP-LC3B expression vectors were co-transfected with pcDNA3/HA (Ctrl) or pcDNA3/HA-CEBPD (CD) expression vectors into Huh7 cells (upper panel). Huh7 cells that were pre-infected with lentiviruses encoding shLacZ (shC) or shCEBPD (shCD) were transfected with GFP-LC3B expression vectors and then treated with Met (5 mM) for 6 h (lower panel). The numbers of LC3B puncta were evaluated under a fluorescence microscope.