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. 2017 Jan 16;8(8):13872–13885. doi: 10.18632/oncotarget.14681

Figure 6. hsa-mir-140 suppresses IL-6 expression induced by RB depletion in human breast cancer cells.

Figure 6

(A) The sequence of the human Il-6 3′UTR and the seed sequence of hsa-mir-140 (underlined) are indicated. mirSVR and Phantcons scores are indicated. (B) RT-qPCR of the indicated genes in MCF7 cells transduced with the indicated vector. N = 3. (C) IB of the indicated proteins in MCF-7 cells transduced with the indicated vector. (D) ELISA of human IL-6 levels in MCF-7 cells transduced with the indicated vector following 8 hours culture. (E) Number of spheres derived from 1.25 × 103 of MCF-7 cells transduced with the indicated vectors. N = 3. (F) Number of spheres derived from 1.25 × 103 of MCF-7 cells transduced with the indicated vector in the presence of indicated concentration of recombinant human IL-6 (#206-IL-010, R&D Systems). N = 3. (G) RT-qPCR of RB7LP in MCF-7 cells transduced with the indicated vector. N = 3. (H) RT-qPCR of hsa-miR-140 in MCF-7 cells transduced with the indicated vector. N = 3. (I) hIL-6-3′UTR WT or hIL-6-3′UTR Mut was transduced into MCF-7 cells together with the hsa-mir-140 expression vector or control (scramble). After 48 hours, luciferase activity was measured.