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. 2017 Jan 25;8(8):13971–13978. doi: 10.18632/oncotarget.14814

Figure 4. The molecular mechanism of PILRLS promote proliferation in RLS cells.

Figure 4

(A) Western-blot to detect the protein level of TCL1A whether regulated by PILRLS. (B) 94T778 cells with stable overexpression of PILRLS or knockdown of PILRLS were treated with protein synthesis inhibitor cycloheximide (CHX, 100 ug/ul) or the proteasome inhibitor MG-132 (50uM) for 24 h. Detect the protein level by western-blot. (C) RT-qPCR was used to detect the mRNA levels of Casp9, BAD, FKHR, P21, P27, MDM2, GSK3B and IKK in knockdown and overexpression PILRLS cells. (D) Luciferase activity of MDM2 in knockdown and overexpression PILRLS cells. (E) Western-blot detect whether the protein level of MDM2 was regulated by TCL1A and PILRLS. (F) CCK-8 assay for verify the function of TCL1A and PILRLS. (G) Western-blot to detect the protein level of TCL1A, AKT, MDM2 and P53 when knockdown and overexpression PILRLS.