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. 2017 Jan 28;8(8):14017–14028. doi: 10.18632/oncotarget.14876

Figure 2. CUDC-907 inhibits c-Myc and HDACs in DLBCL cell lines.

Figure 2

(A) Western blot analysis demonstrating the effect of increasing concentration of CUDC-907 (from 0.01 to 1 μM) on PI3K downstream targets (p4EBP1, pPRAS40 and pS6) and histone H3 acetylation (Ac Histone H3). The actual inhibition of PI3K and HDAC resulted in a decrease in c-Myc protein levels. Activation of caspase 3 and PARP was present as well. (B) Western blot analysis showing the effect of single agent PI3K inhibitor (BKM-120), HDAC inhibitor (panobinostat), or both on PI3K and HDAC targets. The data show that CUDC-907 produces similar results to the combination of BKM-120 and panobinostat. (C) CUDC-907 combines PI3K and HDAC inhibitor into a single scaffold, and therefore is capable of disrupting the oncogenic cooperation between PI3K and c-Myc. (D) MTS assay demonstrating that CUDC-907 inhibits cell proliferation, mimicking the effect of BKM-120 plus panobinostat. Cells were incubated with increasing concentrations of BKM-120, panobinostat and CUDC-907, and viability assessed after 24 hours.