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. 2017 Jan 28;8(8):14017–14028. doi: 10.18632/oncotarget.14876

Figure 3. Quantitative proteomics identifies B-cell receptor signaling proteins as targets for CUDC-907 in DLBCL cell lines.

Figure 3

(A) Summary graph showing fold change cellular proteins that are commonly up or down-regulated in both HBL-1 (blue) and SUDHL-6 (red) cells. (B) A subset analysis of the experiments shown in Figure 3A, demonstrating the effect of CUDC-907 treatment (0.1 μM for 24 hours) on proteins involved in BCR and TLR signaling. Each value represents the average of the fold change (CUDC-907 vs DMSO) obtained in the forward and the reverse experiments. (C) Western blot confirming the effect of CUDC-907 (0.1 μM for 24 hours) on BCR and TLR signaling proteins in B-cell lymphoma cell lines (GCB n = 3 and ABC n = 3). (D) Western blot demonstrating the effect of CUDC-907 (0.1 μM) on MyD88 protein in a panel of ABC DLBCL cells (HBL-1, TMD-8 and OCI-LY-10 harbor the MyD88 mutation L265P). (E) Change in relative mRNA levels of MyD88 over time in B-cell lymphoma cell line panel (GCB n = 3 and ABC = 4) after treatment with CUDC-907 0.1 μM for the indicated time. (F) Western blot demonstrating that CUDC-907 or panobinostat can decrease cellular abundance of MyD88 and BTK in DLBCL cell lines. The analysis was performed after 24 hours of incubation with each drug at the indicated concentration.