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. 2017 Jan 10;8(7):10809–10821. doi: 10.18632/oncotarget.14580

Figure 3. Loss of Gadd45a enhances proliferation and reduces apoptosis of myeloid progenitors expressing BCR-ABL oncoprotein.

Figure 3

A. Analysis of BrDU incorporation in BM cells from mice following BMT with Gadd45a−/−/BCR-ABL and WT/BCR/ABL BM, 35 days post transplantation. Cells undergoing proliferation showed an increase in % S-phase population. B. Quantification of percentage of S phase cells in WT/BCR-ABL and Gadd45a−/−/BCR-ABL BM from A.. Error bars represent SEM, P < 0.05 (n = 4 per genotype). C. Flow cytometric data of apoptotic events (annexin V incorporation) in WT/MIG, WT/BCR-ABL, Gadd45a−/− /MIG and Gadd45a−/−/BCR-ABL BM cells 4 days post retroviral infection. Early apoptotic cells are visualized in lower right quadrant. Data is representative of 3 independent experiments. D. Quantification of percentage of Annexin V positive cells from C.. Summary of FACS data showing average of 3 independent experiments ± SEM P < 0.05.