Figure 1. Gedunin inhibits cell viability of pancreatic cancer cells.

A. C. E. and G. Cells were treated with 5-50μM gedunin (GD). Cell viability was assessed using MTS analysis after treatment with different doses of gedunin for 24h. B. D. F. and H. Control and 25μM gedunin treated cells (their IC₅₀ concentration) were visualized under light microscope at 10X magnification. I. Western blot analysis of total and phosphorylated forms of AKT, PI3K, mTOR, and p70s6kinase in pancreatic cancer cell lysates treated with 25μM gedunin. Data are expressed as the mean ± SEM (*p<0.05) of three separate experiments.