Figure 5. Inhibition of FLT3 D835Y signaling pathways by FLT3 TKI.

BaF3 FLT3 AL mutant cells were treated with lestaurtinib, AG1295 or sorafenib at the indicated concentrations for 1 h. Inhibition of signaling pathways by WB was evaluated in whole cell lysates using antibodies described in Materials and Methods and visualizing bands using enhanced chemiluminescence. Each experiment was repeated at least three times and representative results are shown.