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. 2016 Dec 27;8(7):11053–11062. doi: 10.18632/oncotarget.14278

Figure 5. Otub1-stabilized MDMX contributes to p53 phosphorylation at S46 and apoptosis in response to UV irradiation.

Figure 5

(A) (B) Otub1 promotes UV irradiation-induced apoptosis. T-Rex-U2OS-Flag-Otub1 cells treated without or with 2 ug/ml Dox for 24 hours were subjected to UV irradiation for 6 hours at different doses (A) or 40 J/m2 and harvested at different time points (B). The cells were assayed by IB using indicated antibodies. (C) Otub1 promotes p53-S46P following UV irradiation. T-Rex-U2OS-Flag-Otub1 cells were cultured without or with 2 ug/ml Dox for 24 hours, followed by UV irradiation. The cells were assayed by IB using the indicated antibodies. (D) Knockdown of MDMX attenuates p53-S46P and induction of apoptosis in response to UV irradiation. T-Rex-U2OS-Flag-Otub1 cells infected with scrambled or MDMX shRNA were cultured without or with 2 ug/ml Dox for 24 hours, followed by UV irradiation (40 J/m2) for 6 hours. The cells were then assayed by IB to detect the expression of indicated proteins.