Figure 5. Representative images (40X magnification) of soft agar colonies A. and histogram of colony count B. showing effect of 5-aza 2’ dc treatments on in vitro tumorigenic potential of HK-2 cells exposed to chronic oxidative stress.

OT-HK-2 cells were harvested, plated, and allowed to grow in soft agar as described in materials and methods section and observed microscopically for colony formation and images were captured on Day 14.