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. 2017 Jan 4;8(7):11206–11218. doi: 10.18632/oncotarget.14493

Figure 2. The combination of Rad001 and ATO can synergistically inhibit the colony formation in prostate cancer cells.

Figure 2

For LNCaP cells, they were treated DMSO (control), Rad001 (0.37 μM), ATO (11.19 μM), and their combination, for 24 hrs. For PC3 cells, they were treated DMSO (control), Rad001 (0.35 μM), ATO (12.00 μM), and their combination, for 24 hrs. The cell morphology was detected with the microscopy (A). After the prostate cancer cells treated DMSO (control), Rad001, ATO, and their combination for 14 days, the colony formation was detected (B), and the relative number of colony formation was quantified (C).