Figure 3. EMT reversion by IL-6 Pathway Blockade.
(A) Quantitative PCR detection of the EMT biomarkers mRNA in each cell line. (B) Western-blotting detection of IL-6 pathway inhibition and EMT biomarkers expression change in Eca109 cells exposed to IR, AG490 or sh-Twist. (C) Scratch assay detection of attenuated invasiveness of EMT-Eca109 cells under AG490 or sh-Twist treatment (left) and their quantitative analysis (right). (D) Transwell invasion assay detection of impaired migration and invasion ability of EMT-Eca109 cells under AG490 or sh-Twist treatment (left) and their quantitative analysis (right).