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. 2017 Jan 4;8(7):11268–11283. doi: 10.18632/oncotarget.14499

Figure 4. Involvement of proteases in p45 MET fragment generation.

Figure 4

Sparse (s) and confluent NCI-H1437 cells were treated 16h with various protease inhibitors: (A) with 20 μM pan-caspase inhibitor Q-VD and zVAD. (B) with 1 μM γ-secretase inhibitor Compound E; (C) 16 h with the indicated concentration of the non-permeating cysteine protease inhibitor E64 or the cysteine protease inhibitor EST; (D) with 20 μM cathepsin inhibitor Cati-1 or 20 μM calpain inhibitor ALLN; (E) with the indicated concentration of the cathepsin B inhibitor CatIB2; (F) with the indicated concentration of the calpain inhibitor Calpeptin. (A, B, C, D, E, F) Cell lysates were analyzed by western blotting with an antibody directed against the human MET C-terminal region and GAPDH. (G) Sparse (s) and confluent (conf) NCI-H1437 were lysed and total calpain activity was measured using Suc-LLVY-AMC synthetic substrate in presence or absence of 25 μM of calpain inhibitor ALLN (relative calpain activity to control; n = 3, ±standard deviation).