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. 2017 Jan 4;8(7):11284–11301. doi: 10.18632/oncotarget.14500

Figure 2. Generation and characterization of licMAB molecules.

Figure 2

(A–C, left) Schematic representation of antiCD33 mAb, SIRPα-antiCD33 licMAB, 2xSIRPα-antiCD33 licMAB and the corresponding light chain engineered vectors (VL, variable light chain; CL, constant light chain). (A-C, right) Size exclusion chromatography analyses of the licMABs and mAb. (D) SDS-page analysis of purified SIRPα-antiCD33 licMAB (1), 2xSIRPα-antiCD33 licMAB (2) and antiCD33 mAb (3) under reducing conditions. Bands corresponding to the heavy chain (HC) or light chain (LC) of each molecule are indicated. (E) Melting curves of antiCD33 mAb, SIRPα-antiCD33 licMAB and 2xSIRPα-antiCD33 licMAB, determined by fluorescence thermal shift (RFU, Relative Fluorescence Units). The measured melting points (Tm) of each molecule are specified.