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A. CHRDL2 mRNA expression was measured by QRT-PCR in 9 human CRC cell lines (HCT8, HCT15, HCT116, SW480, SW620, SW403, HT29, LoVo, and CaCo-2). The mRNA level was normalized to 1 for HCT8. B. Protein levels of CHRDL2 in human CRC cell lines were measured by western blot. GAPDH was used as a loading control. The CHRDL2 protein level was normalized to 1 for HCT8.
