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. 2016 Dec 24;8(7):11555–11565. doi: 10.18632/oncotarget.14135

Figure 3. Dexamethasone induces KLF5 transcription via the GR.

Figure 3

A. GR depletion blocks Dex-induced KLF5 expression. Serum-starved (SF) cells were treated with either vehicle control or Dex for 4 h before Western blotting. B. Dex induces KLF5 mRNA expression. HCC1937& HCC1806 cells were serum-starved overnight followed by treatment with 10 μM Dex for the indicated time. KLF5 mRNA expression was measured by qRT-PCR. C. Dex activates the KLF5 promoter through the GR. 293FT cells were transfected with a GR-expression plasmid and the KLF5 promoter luciferase reporter plasmid. One day after transfection, the cells were treated with 10 μM Dex for 12 h. Cell lysates were collected for the dual-luciferase reporter assay. D. GR specifically binds to the KLF5 promoter in vivo, as determined by ChIP assays. The input DNA and water were used as the positive and negative controls, respectively. E. The binding capacity of GR to the KLF5 promoter region was quantified by real-time quantitative PCR. All data shown in this figure were confirmed through independent replicates. *, P<0.05; **, P<0.01; ***, P<0.001.