Table 2. The primer sequences for quantitative real-time polymerase chain reaction.
Gene | Sequence |
---|---|
SDF-1 | F: AAA AGA ATT CAT GAA CGC CAA GGT CGT G |
R: AAA GGT ACC ATC TTG AAC TTG TTT AAA G | |
CXCR4 | F: GGG CTG GAG AGC GAG CAT TGC |
R: ATC GGC GAA GAT GTC GGC CT | |
NGF | F: CGG TCT TCC CGC CCT AGC CTG |
R: ATT TGC ACG CCG CTC CTT TGC | |
BDNF | F: AGA GTG ATG ACC ATC CTT TT |
R: ATC CAC TAT CTT CCC CTT TT | |
GFAP | F: GCT AAT GAC TAT CGC CGC CAA CT |
R: CTC CTT AAT GAC CTC GCC ATC CC | |
GAP-43 | F: GGA GGG AGA TGG CTC TGC TAC |
R: AGT GAC AGC AGC AGG CAC ATC | |
GAPDH | F: AAG GTC ATC CCA GAG CTG AAC GG |
R: ACA ACC TGG TCC TCA GTG TAG CC |
SDF-1, stromal cell-derived factor-1; CXCR4, chemokine receptor-4; NGF, nerve growth factor; BDNF, brain derived neurotrophic factor; GFAP, glial fibrillary acidic protein; GAP-43, growth associated protein-43; qRT-PCR, quantitative real-time polymerase chain reaction; F, forward; R, reverse.