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. 2016 Dec 28;8(7):11708–11718. doi: 10.18632/oncotarget.14315

Figure 3. IMQ or CpG-activated pDCs kill TUBO cells in a TRAIL and Granzyme B-dependent fashion.

Figure 3

A. pDCs were stimulated by IMQ or CpG for 36 hours, and the expression of cytotoxic molecules TRAIL and Granzyme B on pDCs was assessed by flow cytometry and B. release of Granzyme B from pDCs by ELISA. Data shown are expressed as mean ± SEM. C. pDCs were stimulated by IMQ or CpG for 36 hours, and pre-incubated with neutralizing anti-TRAIL, anti-Granzyme B (10 ug/ml each) alone or combined, and isotype-matched control Abs for 30 minutes prior to the addition of TUBO cells. Cytotoxic activity was determined 5 hours later. Specific lysis means of 3 independent experiments performed in triplicates are represented ± SEM. Paired t-test was used for statistical comparison, *P<0.05, *P<0.01.