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. 2016 Dec 28;8(7):11729–11738. doi: 10.18632/oncotarget.14329

Figure 5. Over-expression of Skp2 attenuates YF-18 induced proliferation inhibition, G2/M phase accumulation and migration suppression.

Figure 5

A. A549-luciferase cells were transfected with Skp2 and treated with different concentrations of YF-18 for 24 hours. Western blot assays were involved to detect the expression of skp2, p27, and E-cadherin. B. A549-luciferase cells were transfected with Skp2 and treated with YF-18. Viable cells were detected by MTT assay. C. A549-luciferase cells were transfected with Skp2 and treated with YF-18 for 24 hours. The cells were analyzed by flow cytometry to evaluate the cell cycle distribution. D and E. A549-luciferase cells were transfected with Skp2 and wound healing (D) and transwell (E) assays were conducted to evaluate cell migration. Data is represented as mean ± SD.