Figure 3. Constitutive activation of hypoxic response in HT-29 ρ0x cells.

a. Enrichment plot of “winter hypoxia metagene” [59] from MSigDB c2 curated collections, for genes expressed highly in HT-29 ρ⁰x cells and ρ⁰n cells (both in culture). b. Immunoblot analysis of HIF-1α, and phosphorylation status of proteins involved in mTOR signaling in HT-29 Pt, ρ⁰n and ρ⁰x cells under normoxia. RPS3 was used as a loading control. c, d. HIF-1 DNA-binding ability (c) and HRE-luc activity (d) in HT-29 Pt, ρ⁰n and ρ⁰x cells under normoxia.