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. 2017 Jan 2;8(7):11841–11854. doi: 10.18632/oncotarget.14415

Figure 5. HIF-1 transcriptional activity suppression by mitochondrial inhibitors.

Figure 5

a. OCR of HT-29 Pt cells treated with the indicated concentrations of rotenone (RTN), antimycin A (ANT), oligomycin (OLG), FCCP or DNP. b-e. HRE-luc activity of indicated cells (HT-29, PANC1, 293T and HT1080) after 18 hours of hypoxia (Hyp) or normoxia (Nor), after treating with the indicated concentrations of RTN, ANT, OLG, FCCP or DNP. f. Immunoblot analysis of HIF-1α. HT-29 Pt cells were treated at the indicated times and under hypoxic stress (Hyp) with no inhibitor (control, C) rotenone (R, 100 nM), antimycin A (A, 5 ng/ml), or oligomycin (O, 0.5 μg/ml). mTOR was used as a loading control.