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. 2017 Jan 3;8(7):11990–12002. doi: 10.18632/oncotarget.14467

Figure 5. Autocrine VEGF signaling promoted cell proliferation through a VEGFR2-PLCγ1-ERK1/2 pathway in GC.

Figure 5

A. gastric cancer cells were treated with rhVEGF and were harvested at different time points. The time course changes of the phosphorylation of VEGFR2, PLC, and ERK1/2 were detected by Western blot. B. The proliferation of gastric cancer cells in response to PLCγ1 inhibitor (U73122) in SGC-7901 and BGC-823 cells. C. Blocking PLCγ1 with U73122, proliferation of gastric cancer cells in response to rhVEGF in in SGC-7901 and BGC-823 cells. D. After treating cells with U73122, the protein levels were measured by Western blot. GAPDH was included as a loading control. Mean±SEM, t-test, *P<0.05, **P<0.01, ***P<0.001.