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. 2017 Jan 4;8(7):12003–12012. doi: 10.18632/oncotarget.14479

Figure 2. RCAN1 decreased cell viability in glioma cell lines.

Figure 2

U251 and T98G cells were infected with lentivirus expressing RCAN1 (RCAN1) or negative control (Control) for 72 hrs. A. Western blot was performed to assess the RCAN1 protein level. RCAN1 protein levels were normalized to β-actin. The results present the fold-increase relative to control. B. Quantification of (A). Values represent means ± SE (n = 3),*p <0.05 by Student's t test. C. U251 and T98G cells viability were measured by MTT assay. MTT cell viability was measured by the absorbance at 570 nm (ref: 670 nm) at indicated times after infecting 72 hrs. The values represent the means ± S.E. (n = 3), p<0.05 by two-way ANOVA. D. U251 and T98G cells viability were measured by cell colony formation assay. E. Quantification of (D). Values represent means ± SE (n = 3),*p <0.05 by Student's t test.